Site-directed spin labeling of 2'-amino groups in RNA with isoindoline nitroxides that are resistant to reduction.
نویسندگان
چکیده
Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2'-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by pulsed EPR. After conjugation to RNA, a tetraethyl isoindoline derivative showed significant stability under reducing conditions.
منابع مشابه
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عنوان ژورنال:
- Chemical communications
دوره 51 66 شماره
صفحات -
تاریخ انتشار 2015